MCCOY'S 5A

產(chǎn)品簡(jiǎn)介：1959, McCoy等報(bào)道了體外培養(yǎng)Novikoff Hepatoma細(xì)胞所需的氨基酸。他們?cè)诨九囵B(yǎng)基5A的基礎(chǔ)上通過調(diào)整加入氨基酸的量產(chǎn)生了MCCOY'S 5A的配方。隨后Hsu 和Kellogg 使用這種培養(yǎng)基成功培養(yǎng)了一系列來源于多種組織的原代細(xì)胞，這些組織包括正常骨髓（bone marrow）、皮膚（skin）、牙齦（gingiva）、 睪丸（testes）、小鼠腎臟（mouse kidney）、網(wǎng)膜（omentum）、 腎上腺（adrenal glands）、肺（lung）、脾臟（spleen）、大鼠胚胎（rat embryos）及其它組織。

該產(chǎn)品含有25mM的HEPES。

產(chǎn)品類型：無菌過濾即用型液體培養(yǎng)基

滲 透 壓：280-330 mOsm

酸 堿 度：7.2－7.4

參考文獻(xiàn)：

1. McCoy, T.A., Maxwell, M. and Kruse, P.F., (1959). Amino Acid Requirement of the Novioff Hepatoma In Vitro. Proc. Soc. Exp. Biol. Med. 100, 115-118.

2. Patterson, M.K. and Dell’orco, R.T., (1978). Preparation of McCoy’s Medium 5A. Tissue Culture Association Manual. 4, 737-740.

3. Hsu, T.C. and Kellogg, D.S., (1960). Primary Cultivation and Continuous Propagation In Vitro of Tissues from Small Biopsy Specimens. J.N.C.I. 25, 221-231.

4. Iwakata, S. and Grace, J.T., (1964). Cultivation in Vitro of Myeoblasts from Human Leukemia. New York State Journal of Med. 64, 2279-2282.

5. Morton, H.J., (1970). A Survey of Commercially Available Tissue Culture Media. In Vitro. 6, 89.

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注意：以上文獻(xiàn) 僅供參考

MCCOY'S5A，withL-Glutamine，with25mMHEPES

MCCOY'S5A，withL-Glutamine，with25mMHEPES